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      A platform of genetics and genomics

                             analysis and application in Nicotiana



       Primer3 is a free online tool to design and analyze primers for PCR and real time PCR experiments. Primer3 can also select single primers for sequencing reactions and can design oligonucelotide hybridization probes. The online tool constitutes some important features like primer detection, cloning, sequencing and Primer listing.



Please inoput the sequences(in FASTA):

(The sequence from which to choose primers,The sequence must be presented 5' to 3')





Advance parameters:


Min-TM: 55.0
Max-TM: 60.0
Min-GC: 40.0










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Please contact: Long Yang