The flowchart of the GMGM

 

A special method and several softwares were used in this study, the flowchart as follows: firstly, using a blast to find the match and mismatch sequences of two types of etiology;secondly, used a series of Perl scripts to find the different sequences between the two types of etiology; thirdly, based on these sequences, the primers were designed and an analysis was also done among the five strains; at last, the primers was verified by the experiment.