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Ninety-six NIX markers were used for experimental validation by PCR amplification on a testing panel of five Nicotiana accessions, including N. benthamiana(B), N. tabacum K326(K), Nicotiana glutinosa(G), N. tomentosiformis(T) and N. sylvestris.

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N.ben &N.K326 || N.glu & N.tom || N.syl

N. benthamiana(B) and N. tabacum K326(K)

Nicotiana glutinosa(G) and N. tomentosiformis(T) top

N. sylvestris top

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