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Table 1 A portion of the table showing amplification information for NIX markers in the seven Nicotiana genomes

Marker_ID N. ben N. syl N. tom N. oto N. tab TN90 N. tab K326 N. tab BX Polymorphism Alleles
>NIX189 NA 347 NA NA 347 347 347 No 1
>NIX190 697 232 NA NA 234 234 234 Yes 3
>NIX191 NA NA NA NA 179 179 179 No 1
>NIX192 NA 285 NA NA 287 287 287 Yes 2
>NIX193 155 157 155 157 155+157 155+157 155+157 Yes 2
>NIX194 NA 816 NA NA NA 233 NA Yes 2
>NIX195 NA NA NA NA 202 215 201 Yes 3
>NIX196 NA 208 NA NA 207 207 207 Yes 2
>NIX197 NA 286 NA NA 286 286+287 286 Yes 2
>NIX198 NA 128+278+308+338 NA NA 128 128 128 No 1
>NIX199 NA  278+308+338 NA NA 278+308 278+308 278+308 Yes 3

Figure 1 . Frequency distributions of different types of SSR repeat units in Nicotiana genomes

Figure 2 The localization of SSR in Nicotiana genome sequences

Figure 3. Venn diagram showing the number of common and specific NIX markers in Nicotiana species and varieties

Figure 4 Experimental validations of NIX markers by amplification and allele scoring

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